Laboratory for Biomaterials and Tissue Engineering

• Comparative Transcriptomic and Phenotypic Analysis of Induced Pluripotent Stem Cell Hepatocyte-Like Cells and Primary Human Hepatocytes, Gandhi N, Wills L, Akers K, Su Y, Niccum P, Murali TM and Rajagopalan P, Cell and Tissue Research, in press, 2024
• Law JN, Orbach SM, Weston BR, Steele PA, Rajagopalan P, and Murali TM, Computational Construction of Toxicant Signaling Networks, Chem. Res. Toxicol., 36, 8, 1267-1277, 2023.
• Gandhi N, Inzana T, and Rajagopalan P, Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease, ACS Infectious Diseases, 9(6), 1168-1179, 2023.
• Hatlen RR and Rajagopalan P, Investigating Trans-differentiation of Glioblastoma Cells in an In Vitro 3D Model of the Perivascular Niche, ACS Biomaterials Science and Engineering, 9, 6, 3445-3461, 2023.
• Law JN, Akers K, Tasnina N, Della Santina CM, Deutsch S, Kshirsagar M, Klein-Seetharaman J, Crovella M, Rajagopalan P, Kasif S, and Murali TM, Interpretable Network Propagation with Application to Expanding the Repertoire of Human Proteins that Interact with SARS-CoV-2, GigaScience, 10, giab082, 2021
• Hatlen RR and Rajagopalan P, Environmental interplay: Stromal cells and biomaterial composition influence in the glioblastoma microenvironment, Acta Biomaterialia, 132, 421-436, 2020.
• Wills LR and Rajagopalan P, Advances in Human Induced Pluripotent Stem Cell-derived Hepatocytes for use in Toxicity Testing, Annals of Biomedical Engineering, 48, 1045-1057, 2020.
• Kothari A and Rajagopalan P, The assembly of integrated rat intestinal-hepatocyte cultures, Bioengineering and Translational Medicine, 5, e10146, 2020.
• Ford AJ, Orbach SM and Rajagopalan P, Fibroblasts Stimulate Macrophage Migration in Interconnected Extracellular Matrices through Tunnel Formation and Fiber Alignment, Biomaterials, 209, 88-102, 2019.
• Kothari A and Rajagopalan P, Isolating Rat Intestinal Explants for In Vitro Cultures, Current Protocols in Toxicology, 80, e79, 2019.
• Ford AJ and Rajagopalan P, Measuring Cytoplasmic Stiffness of Fibroblasts as a Function of Location and Substrate Rigidity Using Atomic Force Microscopy, ACS Biomaterials Science & Engineering, 4, 3974-3982, 2018. Selected to be featured in ACS Editors' Choice.
• Orbach SM, Ford AJ, Saverot SE, and Rajagopalan P, Multi-Cellular Transitional Organotypic Models To Investigate Liver Fibrosis, Acta Biomaterialia, 82, 79-92, 2018.
• Tegge AN, Rodrigues RR, Larkin AL, Vu L, Murali TM, and Rajagopalan P, Transcriptomic Analysis of Hepatic Cells in Multicellular Organotypic Liver Models Scientific Reports, 8, 11306, 2018.
• Orbach SM, Ehrich MF, and Rajagopalan P, High-Throughput Toxicity Testing of Chemicals and Mixtures in Organotypic Multi-Cellular Cultures of Primary Human Hepatic Cells, Toxicology in Vitro, 51, 83-94, 2018.
• Ford AJ and Rajagopalan P, Extracellular Matrix Remodeling in 3D: Implications in Tissue Homeostasis and Disease Progression, Wiley Interdisciplinary Reviews - Nanomedicine and Nanobiotechnology, 10, e1503, 2018.
• Vu LT, Orbach SM, Ray K, Cassin ME, Rajagopalan P, and Helm RF, The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment, Proteome Science, 15, 2017.
• Orbach SM, Cassin ME, Ehrich MF, Rajagopalan P, Investigating acetaminophen hepatotoxicity in multi-cellular organotypic liver models, Toxicology in Vitro, 42, 10-20, 2017.
• Orbach SM, Less RR, Kothari A, and Rajagopalan P, In Vitro Intestinal and Liver Models for Toxicity Testing, ACS Biomaterials Science and Engineering, 3, 1898-1910, 2017.
• Cassin ME, Ford AJ, Orbach SM, Saverot SE, and Rajagopalan P, The Design Of Antimicrobial LL37-Modified Collagen-Hyaluronic Acid Detachable Multilayers, Acta Biomater., 40, 119-129, 2016.
• Ford AJ, Jain G, and Rajagopalan P, Designing a Fibrotic Microenvironment to Investigate Changes in Human Liver Sinusoidal Endothelial Cell Function, Acta Biomater., 24, 220-227, 2015.
• Jain G, Ford AJ, and Rajagopalan P, Opposing Rigidity-Protein Gradients Reverse Fibroblast Durotaxis, ACS Biomater. Sci. Eng., 1 (8), 621-631, 2015.
• Vu LT, Jain G, Veres B, and Rajagopalan P, Cell Migration on Planar and 3D Matrices: A Hydrogels-Based Perspective, Tissue Engineering Part B: Reviews, 21, 67-74, 2015.
• Cassin ME and Rajagopalan P, Polyelectrolyte Multilayers for Applications in Hepatic Tissue Engineering, invited book chapter in "Layer-by-Layer Films for Biomedical Applications", Picart C, Caruso F, and Voegl J (eds.), Wiley-VCH Verlag GmbH and Co., 2014.
• Vu LT, Less RR, and Rajagopalan P. The promise of organotypic hepatic and gastrointestinal models, Trends in Biotechnology, 32(8), 406-413, 2014.
• Larkin AL, Rodrigues RR, Murali TM, and Rajagopalan P. Designing a Multi-cellular Organotypic 3D Liver Model with a Detachable, Nanoscale Polymeric Space of Disse, Tissue Engineering Part C: Methods, 19(11), 875-884, 2013.
• Lasher CD, Rajagopalan P, and Murali TM. Summarizing Cellular Responses Using Biological Process Networks, BMC Systems Biology, 7, 68, 2013.
• Rajagopalan P, Kasif S, and Murali TM. Systems Biology Characterization of Engineered Tissues, Annual Reviews of Biomedical Engineering, 15, 55-70, 2013.
• Evans DW, Vavvalle N, DeVita R, Rajagopalan P, and Sparks JL. Nano-Indentation Device for Investigating the Biomechanics of Liver Extracellular Matrix, Experimental Mechanics, 53(2), 217-229, 2012.
• Evans DW, Rajagopalan P, Devita R, and Sparks JL. Compression instrument for tissue experiments (CITE) at the meso-scale: device validation, Biomed Sci Instrum, 47, 264-9, 2011.
• Detzel CJ, Larkin AL, and Rajagopalan P. Polyelectrolyte Multilayers in Tissue Engineering, Tissue Engineering Part B Reviews, 17(2), 101-113, 2011.
• Detzel CJ, Kim Y, and Rajagopalan P. Engineered 3D Liver Mimics Recapitulate Critical Rat-Specific Bile Acid Pathways, Tissue Engineering Part A, 17(5-6), 677-689, 2011.
• Lasher CD, Rajagopalan P, and Murali TM. Discovering Networks of Perturbed Biological Processes in Hepatocyte Cultures, PLoS One, 6(1), e15247, 2011.
• Kim Y and Rajagopalan P. 3D Hepatic Cultures Simultaneously Maintain Primary Hepatocyte and Liver Sinusoidal Endothelial Cell Phenotypes, PLoS One, 5(11), e15456, 2010.
• Kim Y, Lasher CD, Milford LM, Murali TM and Rajagopalan P. A Comparative Study of Genome Wide Transcriptional Profiles of Primary Hepatocytes in In vitro Cultures, Tissue Engineering Part C Methods, 16(6), 1449-1460, 2010.
• Larkin AL, Davis RM, and Rajagopalan P. Biocompatible, Detachable, and Free-standing Polyelectrolyte Multilayer Films, Biomacromolecules, 11(10), 2788-2796, 2010.
• Kim Y, Larkin AL, Davis RM and Rajagopalan P. The Design of In Vitro Liver Sinusoid Mimics using Chitosan-Hyaluronic Acid Polyelectrolyte Multilayers, Tissue Engineering Part A, 16(9), 2731-2741, 2010.
• Hale NA, Yang Y, and Rajagopalan P. Cell Migration at the Interface of a Dual Chemical-Mechanical Gradient, ACS Applied Materials and Interfaces, 2(8), 2317-2324.
• Hajicharalambous CS, Lichter J, Hix WT, Swierczewska M, Rubner MF, and Rajagopalan P. Nano- and Sub-micron Porous Polyelectrolyte Multilayer Assemblies: Biomimetic Surfaces for Human Corneal Epithelial Cells, Biomaterials, 30(23-24), 4029-36, 2009.
• Coulter, JP, Rajagopalan P, Gomatam, R, and Kim, Y. The Effects of Dynamic Polymer Melt Manipulation of the Bioinert and Biodegradable Performance of Injection Molded Biomedical Products. ANTEC Proceedings of the 66th Annual Technical Conference, Society of Plastics Engineers, 523-527, 2008.
• Coulter, JP, Rajagopalan P, Gomatam, R, and Kim, Y. Realization of Polymer-Based Nanostructured Surfaces for Biomedical Applications via Nanoscale Injection Molding. ANTEC Proceedings of the 66th Annual Technical Conference, Society of Plastics Engineers, 2489-2493, 2008.
• Swierczewska M, Hajicharalambous C, Janorkar AV, Megeed Z, Yarmush ML, and Rajagopalan P. Cellular Response to Elastin Like Polypeptide Multilayers. Acta Biomaterialia, 4(4), 827-837, 2008.
• Janorkar AV, Rajagopalan P, Yarmush ML, and Megeed Z. ELP-Polyelectrolyte Structure Confers Control Over Hepatocyte Morphology and function. Biomaterials, 29(6), 625-632, 2008.
• Rajagopalan P, Shen CJ, Berthiaume F, Tilles AW, Toner M, and Yarmush ML. Polyelectrolyte Nano-scaffolds for the Design of Layered Cellular Architectures. Tissue Engineering , 12(6), 1553-1563, 2006.
• Rajagopalan P, Berthiaume F, Tilles AW, Toner M, and Yarmush ML. Selective Enhancement of Cytochrome P450 Activity in Rat Hepatocytes by In Vitro Heat Shock. Tissue Engineering, 11(9-10), 1527-1534, 2005.
• Rajagopalan P, Marganski W, Brown XQ, and Wong JY. Direct Comparison of the Spread Area, Contractility, and Migration of balb/c 3T3 Fibroblasts Adhered to Fibronectin- and RGD-Modified Substrata, Biophysical Journal, 87(4), 2818-2827, 2004.
• Zaari N, Rajagopalan P, Kim SY and Wong JY. Photopolymerization in Microfluidic Gradient Generators: Microscale Control of Substrate Compliance to Manipulate Cell Response. Advanced Materials, 16(23-24), 2133-2137, 2004.
• Revzin A, Rajagopalan P, Tilles AW, Berthiaume F, Yarmush ML. and Toner M. Designing a Hepatocellular Microenvironment with Protein Microarraying and Poly(ethylene glycol) Photolithography. Langmuir, 20(8), 2999-3005, 2004.
• Wong JY, Velasco A, Rajagopalan P, and Pham Q. Directed Movement of Vascular Smooth Muscle Cells on Gradient-Compliant Hydrogels, Langmuir, 19(5), 1908-1913, 2003.
• Zaari N, Rajagopalan P, Kim SY, and Wong JY. Microgradient mechanically-compliant polyacrylamide gels to probe cell response. Polym. Prepr. (Am. Chem. Soc., Polym. Chem.), 44(1), 200-201, 2003.
• Rajagopalan P, Marganski W, Dembo M, and Wong JY. Traction Stresses and Morphology of 3T3 Fibroblast Cells on Fibronectin- versus RGD-Modified Elastic Substrata. Mat. Res. Soc. Symp. Proc., Advanced Biomaterials, 711, 231-237, 2002.
• Rajagopalan P and McCarthy TJ. Polymer-Metal Nanocomposites in Supercritical Fluid Carbon Dioxide.Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 40, 228, 1999.
• Rajagopalan P and McCarthy TJ. Two–Step Surface Modification of Chemically Resistant Polymers: Blend Formation and Subsequent Chemistry. Macromolecules, 31(15), 4791-4797, 1998.
• Rajagopalan P and McCarthy TJ. Surface Modification of Poly(tetrafluoroethylene) Using Supercritical Carbon Dioxide Processing. Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 38, 678, 1997.
• Rajagopalan P, Wang Y, and Risen WM. Spectral Studies of Novel Lanthanide Containing Polymers by Near Infrared and FT-Raman Spectroscopy. J. Mol. Structure, 405(1), 59-66, 1997.
• Rajagopalan P, Tsatsas A, and Risen WM. Synthesis and Near Infrared Properties of Rare Earth Ionomers. J. Polym. Sci., Part B, Polym. Phys. Ed. 34(15), 151-161, 1996.
• Biswas A, Rajagopalan P, and Risen WM. Morphology of Samarium Neutralized Ethylene Ionomers. Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 37, 422, 1996.
• Rajagopalan P, Kim JS, Brack HP, Lu X, Weiss RA, Eisenberg A, and Risen WM. Molecular Interpretation of Miscibility in Polyamide-6 blends with Alkali Ionomers of Sulfonated Polystyrene. J. Polym. Sci., Part B, Polym. Phys. Ed., 33(3), 495-503, 1995.
• Bhattacharjee S, Rajagopalan P, Bhowmick AK, and Avasthi BN. Selective Hydrogenation of Olefinic Bonds in Styrene-Isoprene-Styrene Triblock Copolymer by Palladium Acetate Catalyst. J. Appl. Polym. Sci., 49(11), 1971-1977, 1993.

PATENTS

• Rajagopalan P, Campbell JR, and Lewis LN. Flame-retardant polycarbonate-silsesquioxane compositions, methods for making and articles made thereby. USPTO Patent No. 6518357, Assignee General Electric Co., 2003.